The aim of this study is to measure the bio-environmental effects on the development of mass-tourism Tameshkelvillage (Tonekabon) located on the north of Iran. This study measures the attitude of the host and guest community and the objective documents. The area of the present study covers 18 villages,2481 households, and 9024 people who live in Neshtai district in Tonekabon.The method used for this study is a survey with random sampling.More than 190 households lived in 9 sample villages and 180 tourists responsed to the questionnaires. The data were gathered and analyzed by parametric statistic tests. The results show that the development of tourism and the poor management in the local villages have negative effects onthe natural resources destruction, the misusage of farmland and gardens and the increase of environmental pollutions. The results also clear that based on the attitudes of host and the guest comunition and the objective documents and lack of effective programings caused negative results on the bio-environmental tourism in the area.