One of the main roles of universities is entrepreneurship culture development among students. Accordingly, the aim of this study was investigating influence of internal factors in university on the entrepreneurial culture development among agricultural students of Hamedan Universities. This descriptive research has done by survey method and questionnaire instrument. Research population were included agricultural students of Bu-Ali Sina, Payam Noor and Applied Science Universities (640 students) that selected 160 students as study sample based on Cochran formula. Findings showed that variable suitable creating conditions only have not significant effect among seven factors of internal factors in university. Also, creativity culture had the most impact on entrepreneurial culture. Also, conceptual model of study was good fitness. In finally, according to results, some recommendations have presented at the end of article.